Sartorius (ForteBio) Octet .frd

Octet biolayer-interferometry instruments write one .frd result file per biosensor. OpenReadout returns the sensor’s wavelength shift against time through every assay step, the step table and the instrument facts. Kinetic fits are not in these files.

Derived from public files of two depositors (OctetRED96e and OctetRED384, DataAcquisition 11.1), compared value by value with pykingenie (MIT) run as a black box. Provenance: docs/provenance/sartorius-octet.md. Crate: openreadout-biophys.

format id	files	reads	confidence
sartorius-octet-frd	.frd result files of Octet biolayer-interferometry instruments (one per biosensor)	the sensor’s wavelength shift against time through every assay step, the step table (sample, well type, concentration, molar concentration, molecular weight, temperature, times, shake speed, status), instrument model and serial, acquisition software, operator, start time, sensor type and role	generated (book/src/reference/evidence.md)

Not read: kinetic fits (made by the analysis software; not in .frd files); non-kinetics experiments (no KineticsData: refused with exit 6); the experiment method (.fmf) and plate definition files next to the .frd files.

Layout

UTF-8 XML, root ExperimentResults.

element	content	our reading
ExperimentInfo	RTDVersion, RunID, ExperimentType/ExperimentSubType, StartDateTime (local), MachineName, UserName, PlateName, SensorName, SensorPlate, SensorType, SensorRole, WritingSW, InstrumentType, InstrumentSerial, InstrumentFW, IntegrationTime, DSPType, DSPStitching, HasManifest	experiment facts; vendor tree octet; RTDVersion → format_version
KineticsData/Step	CommonData (SampleLocation, SampleID, SampleGroup, SampleInfo, SampleRow, SamplePlate, WellType, Concentration, ConcentrationUnits, MolarConcentration, MolarConcUnits, MolecularWeight, Temperature, StartTime, AssayTime), FlowRate, StepType, StepName, StepStatus, ActualTime, CycleTime	table 0 steps (−1 → not set)
Step/AssayXData, Step/AssayYData	base64 of little-endian float32: time (s, continuous across steps) and wavelength shift (nm); Points = the value count	trace 0

What the reader returns

• Trace 0 (sensor <SensorName>): channels time (s; the irregular axis), response (nm), step (1-based step of each point): every step’s values concatenated in file order.
• Table 0 steps: step, name, type, status, sample_id, well_type, sample_row, sample_location, concentration with concentration_unit, molar_concentration with molar_concentration_unit, molecular_weight, temperature (°C), start (s, the step’s first time), assay_time, actual_time, cycle_time (s), flow_rate (rpm), points.
• Experiment: vendor Sartorius (ForteBio), model, serial, software version (WritingSW), operator, start time, method (KINETICS KBASIC), sensor, sensor type and role, plate name, duration.
• check: every step’s arrays decode to their stated point count; a step that starts before the previous one ends is a warning.

Validation

cargo test -p openreadout-corpus-tests --features corpus (tests/series_oracle/mod.rs, oracle/octet_oracle.py, pykingenie run as a black box): 256 sampled response and time values, every step’s point count, concentration, molar concentration, molecular weight, temperature, assay, actual and cycle time and shake speed, and the model, serial, operator and start time.

Vocabulary (public API of openreadout-biophys, Octet)

identifier	meaning
OctetReader	reader of Sartorius Octet .frd files
OCTET_FORMAT_ID	sartorius-octet-frd

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How this reader was derived, file by file: provenance log.